; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. Contact Us Phone: +1. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. 4. Ease of use. 蛍光 発光 ELISA. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. 在SpectraMax i3x和SpectraMax iD5微孔板读板机上使用 SoftMax Pro软件绘制标准曲线，并进行4参数曲线拟合(图 4)，结果见表2。使用EC50评估该测定方法的适用性，EC50值 应低于150 nM。对于两个读板机，EC 50值均低于85 nM，处 于可接受范围内。Cisbio将可接受的信噪比定义为≥. Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers. Afterward, the absorbance was measured at 450 nm on a Microplate Reader (Spectra Max i3x, Molecular Devices, USA). Register to download your free eBook today. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Microplate optimization should be performed the first time the assay is run, and subsequently for . The SpectraMax ® i3x. 0´ 104 8. User-upgradeable detection modules allow you to expand the capabilities. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. 7 mm for all shake intensities. Description 1. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Contact our support team with your questions regarding Molecular Devices products and applications. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Manufacturers try to design their instruments so they can be. Multimode Reader Comparison Chart. CRITICAL STEP Avoid air bubbles; reverse pipetting the luciferase reagent into the plate is a good way to achieve this. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Other services inlcude but are not limited to anti-drug activity (ADA), PK/PD assays as well as antibody screenings. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. Cell viability was calculated as average percent of the OD reading relative to that of control group, which was normalized to 100%. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Accessories for SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. Immunofluorescence StainingAfter cooled down to room temperature, the concentrations of the solution were measured by microplate reader (Spectra Max i3x) at 490 nm (Kagi and Schaffer, 1988). The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Red and green fluorescence was then measured (emission at 590 and 530 nm, respectively, with excitation at 490 nm) using a fluorescence microplate reader (Spectra Max i3x, Molecular Devices Inc. Thetypeof. Amplitude : 1. Description 1. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. We would like to show you a description here but the site won’t allow us. Find regional support. ATP (“flash” luminescence using . INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe absorbance was recorded on a spectra Max i3x microplate reader (Molecular Devices, USA) at 450 nm. The MiniMax cytometer with SoftMax® Pro Software can be used to image and analyze a variety of cells. No Stain Analysis: Eliminate cell staining for cell counting and confluence measurements using. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Remove the transport bracket and store the retaining screws in the bag that held the 3. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. 価格、見積もり、お近くの販売店を. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. 발광 (Luminescence) 리더기. , 2011). Spectranet customer portalThe SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. The SpectraMax i3 also allows the user to identify antibodies during Elisa Assays. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 99. 99（图一）。After incubation at room temperature for one hour in the dark, we measured the fluorescence intensity using a SpectraMax i3x (Molecular Devices, CA, USA), with excitation at 350 nm and dual emissions at 435 nm and 490 nm. com. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. 303 unable to cal dark current. Expand your lab’s capabilities to include flash applications such as dual luciferase and. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. User-upgradeable applications The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Bioz Stars score: 98/100, based on 1 PubMed citations. To learn more about the device, please watch this short video. Find regional support. The treatments were. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 1) Select the correct plate and the wells that you want to measure. Find regional support. 303 unable to cal dark current. Microplate Stacker. • Removing the transport locks from the PMT shutters and drawers. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeSpectraMax MiniMax 300 イメージングサイトメーター は、研究室でのアップグレードが可能なSpectraMax i3xシステム専用のオプションで、ユーザーは複雑なイメージングシステムに投資することなく、細胞増殖を解析できます。SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. See SpectraMax® Microplate Reader User Manual Download Page. Dependingontheapplication,theinstrumentcanread6,12,24,48,96,and384-well microplates. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5e Fluorescence was quantified using a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) with an excitation and emission wavelength being 365 nm and 450 nm, respectively (Jian et al. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . D 1 1. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. SoftMax Pro GxP Compliance Software. 0´ 104 4. Explore a wealth of applications with the future-ready SpectraMax i3x. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. Figure 4. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. Increased fluorescence performance. com The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. 635. 001 0. Multimode Reader Comparison Chart. Spectranet customer portalPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. i3x. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Contact Local Team. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Published Dec 16, 2020 | Updated Dec 16, 2020. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. It allows you to explore cellular pathways and protein activation and expression in one system. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™. 0. ValitaTiter Plus settingsThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. 3. TUNE Tunable Wavelength Detection Cartridge. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . 4 / 5. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. The SpectraMax i3 is specifically used in this lab for BCA assays and Elisa Assays. 06, 0. , 2021). 4 mg mL −1; 1. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Introduction. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. Note: Additional readers with similar performance are listed. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthTNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. Nucleic Acid and Protein Quantitation. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 190 control panel are shown in boxed Helvetica type. Promega. 2. , 1997). Description 1. Relative mitochondrial polarization was expressed as the ratio of emission at 590 nm to that at 530 nm (Shin et al. SpectraMax i3x. Published Dec 16, 2020 | Updated Dec 16, 2020. 305 reference level saturation. The SpectraMax i3x reader provides the. Also refer to this Multimode Reader Comparison Chart. 1. 1. Home > Search Results > Danaher Inc > spectramax i3x. Actual shake speed is based on the microplate format. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. The chemical reduction of resazurin was evaluated incubating each well of the microtiter plate with 10 μL (10% of final well volume) of 0. Introduction. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable. In dual reporter gene assays, cells are transfected with two vectors, the first containing an experimental reporter gene coupled to a regulated promoter of interest, andSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strength*On the SpectraMax i3x reader only. The fluorescence spectra were acquired using a microplate reader (Spectra Max i3x; Molecular Devices, USA). Find regional support. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. 2 only) SpectraMax M-series Microplate readers. infoboxeu@moldev. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataBased on the SpectraMax i3x system, ACROBiosystems provides biomedical research and development services for customers seeking quantification and detection of antibodies-antigen interaction. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Examples: fiPress carefully to engage,fl fiDo not press down. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Example: [Drawer]. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. Research Article Streptococcus lutetiensis Induces Autophagy via Oxidative Stress in Bovine Mammary Epithelial Cells Peng Chen ,1 Jingyue Yang,1 Naiwen Wu,1 Bo Han,1 John P. The SPECTRAmax 340PC can mea- Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. 吸収度マイクロプレートリーダー SpectraMax® Mini. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. Features. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Page 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. Formicro-volumemeasurements,theinstrumentsupportsSpectraDrop24-wellSpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. SpectraMax i3x Multi-Mode Detection Platform User Guide. Increased fluorescence performance. It also integrates with the. spectramax i3x (Danaher Inc)The fluorescence emission intensity spectra were recorded using a microplate reader (Spectra Max® i3x, Molecular Devices, LLC. 在SpectraMax i3x和SpectraMax iD5微孔板读板机上使用 SoftMax Pro软件绘制标准曲线，并进行4参数曲线拟合(图 4)，结果见表2。使用EC50评估该测定方法的适用性，EC50值 应低于150 nM。对于两个读板机，EC 50值均低于85 nM，处 于可接受范围内。Cisbio将可接受的信噪比定义为≥. Simplified workflow: Add cellular imaging assays in a small footprint. Description 1. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. . Figure 4. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. Synthesis of NORP Synthesis of compound 1. Download PDF. These concentrations are then used to perform western blots. Published Dec 16, 2020 | Updated Oct 10, 2023. Overview of SpectraMax MiniMax 300 Imaging Cytometer. SpectraMax Microplate Readers. detection cartridge LUM96: 59. Analysis of HTRF assays involves Cisbio’s patented ratiometric reduction method based on the two emission wavelengths detected. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. Bioz Stars score: 98/100, based on 1 PubMed citations. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Hands-on fun and learning that empowers exploring, inventing and sharing. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. Instrument. 0. Bioz Stars score: 98/100, based on 1 PubMed citations. flThe SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The assay window spanned four orders of magnitude. 200-800 nm using Spectra Max i3X microplate reader. ZERO BIAS - scores, article reviews, protocol conditions and more. SpectraMax M 시리즈 Microplate Reader, Cuvette Port, IQ OQ 검증 | Molecular Devices. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Replicate values were averaged and imported into Design-Expert 8. After incubation at 37 °C and 5% CO 2 for 20 min, fluorescence images were taken using a Spectra Max i3x with MiniMax 300 Imaging Cytometer (Molecular Devices, San Jose, CA, USA) with the excitation and emission setup provided with SoftMax Pro 7. SeeSpectraMax MiniMaxImagingCytometeronpage85. Structured Review. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. Robust,. SpectraMax i3x读板机针对96孔板和384孔板进行检测时，最低检测下限可至10细胞每孔。 此种检测方法的灵敏度远远高于标准比色法和绝大多数荧光法。 CellTiter-Glo试剂检测线性范围覆盖所有细胞浓度区间，动态学检测范围大于3个数量级且r2 > 0. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Sensitive TRF detection of two simple, mix-and-measure assay workflows. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Description 1. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. . Myeloperoxidase (MPO) Assay. Fluorescence at 365 nm excitation and 450 nm emission filters was determined based on a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA). How to properly unlock iD series instrument. ATP (“flash” luminescence using . Find regional support. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. i3x. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. 314 low lamp level warning. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). i3. The information below applies to a standard 96 well plate, set to a linear shake mode. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Spectranet customer portalThe Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Select PMT gain o PMT gain = energy used by detector to collect signal o Low gain for samples with high expected signalSpectraMax i3x Multi-Mode Microplate Reader. Its dual light sources, a xenon lamp and LEDs, illuminates samples with incomparable signal strength and superior sensitivity. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. SpectraMax i3x. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. The cell imaging was captured on Zeiss LSM 980 confocal microscopy. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Generate more data points without the need to dilute. Item SpectraMax i3x Multi-Mode Microplate Readers; Company Molecular Devices LLC; Catalog Number SpectraMax i3x <Disabled> This product is no longer available on Biocompare. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely low light. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks iD5. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. To detect physical stability, Pdots were treated with water and incubated with plants. Flow cytometry was used to measure ROS concentrations, based on fluorescence intensity (FL-1, 530 nm) of 10,000 cells. Supplier: Molecular Devices. Molecular Devices Corporation. 11. Here, we demonstrate the benefits of using the SpectraMax ® i3x Multi-Mode Microplate Reader with the SpectraMax ® MiniMax™ 300 Imaging Cytometer in combination with MetaMorph ® Microscopy Automation and Image Analysis Software for performing automated neurite outgrowth imaging and analysis. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. 12911151) 0. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. D. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. 蛍光 発光 ELISA. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 1321N1, a human astrocytoma cell line. Time-. 2 in. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. Price from $9. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. Multimode Readers Comparison Chart. 6 in. Videos & Webinars. SpectraMax Quant dsDNA Assay Kits. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. no. A: percent GFP-positive cells calculated using the classification feature in the software. 2 (Molecular Devices, San Jose, CA). The SoftMax Pro Software provides integrated instrument control. Recommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. The fluorescence quantum yield (QY) of the Pdots was measured by a UV−NIR absolute PL QY. Fluorescence Microplate Reader Comparison Chart. 6% for the on-board optics (monochromators). The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 2C). The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. applications, including dual luciferase and ATP assays. 99（图一）。The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. SpectraMax® i3x Multi-Mode Microplate Reader with MiniMax™ imaging cytometer Integrated multi-mode microplate reader and imaging system • Ideal for cell counting, label-free imaging, and cell proliferation • Western blot, imaging, and injectors on one configurable microplate reader • Stain free, automated cell countingusing the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. ZERO BIAS - scores, article reviews, protocol conditions and more. Winnersh Triangle. Dilution series of HeLa cells transfected with firefly (red plot) and Renilla (blue plot) luciferases. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. 10, and 0. Description 1. 5 decades, with sensitive detection of cells. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. SoftMax Pro Software enables users to quickly set up analysis of cell counts via a simple point and click user interface. 흡광 (Absorbance) 리더기. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the datathe SpectraMax i3x Multi-Mode Microplate Reader with SpectraMax Injector Cartridge Introduction Reporter gene assays are used to study the expression of eukaryotic genes. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataThe SpectraMax i3x reader and MiniMax cytometer can be used with FluoroBlok cell culture inserts to directly count cells from high-quality images to measure cellular migration over time. SpectraMax i3x读板机针对96孔板和384孔板进行检测时，最低检测下限可至10细胞每孔。 此种检测方法的灵敏度远远高于标准比色法和绝大多数荧光法。 CellTiter-Glo试剂检测线性范围覆盖所有细胞浓度区间，动态学检测范围大于3个数量级且r2 > 0. Unlock your i3/i3x instrument after shipment. Prepare your i3/i3x instrument for shipment. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. Explore a wealth of applications with the future-ready SpectraMax i3x. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). (SpectraMax i3/i3x) CatchPoint SimpleStep ELISA (SpectraMax Paradigm) SpectraDrop. Carbonyl assay The plates for carbonyl detection were prepared as described. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Sunnyvale, CA, USA) with excitation at 475 nm. Singlet oxygen (1 O 2) generation was determined through 1,3-diphenylisobenzofuran (DPBF) quenching. Methods. flValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. HeLa cells were plated at 15,000 cells per well in 100 µL of medium in a 96-well black, clear-bottom microplate. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. Molecular Devicesの全商品を見る. Pick the best speed, sensitivity, and price for your needs. Fluorescence emission spectra were collected on an ELISA molecular system (Spectra Max i3x). Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . Table 1. 305 reference level saturation. Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. An MPO assay has been performed to quantify the inflammation reduced by the GAR-PLGA-ES100 NPs in the inflamed cells . SpectraMax i3x Multi-Mode Detection Platform. Read height should be optimized for . Supplier Page. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Bookings are free, but you will need to use iLab to book. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. 1. 1 mg/mL density of resazurin, protected of the light, incubated for 1 h at room temperature. Published Dec 16, 2020 | Updated Dec 16, 2020. Briefly, after exposed to ZnO/GO and Cd, cells were collected and washed with ice-cold PBS twice and then re-suspended in 200 μL binding buffer. This way you’ll save time on finding the necessary info. Prime function 20 amol. To read HTRF®, the SpectraMax i3x® must be first equipped with the SpectraMax i3x® Cisbio HTRF® cartridge, which enables the simultaneous measurement of both 620nm donor and 520 nm acceptor emissions.